Keywords
Gyps Vultures
Red-Headed Vulture
Gastrointestinal Microflora
Respiratory Microflora.
How to Cite
Abstract
Long-term captivity is known to alter the microflora of animals. This alteration in microflora could be because of diet or the environment, and might affect the normal physiological functions of the animal. This study was conducted to assess whether captivity had altered the microflora of the critically endangered Gyps vultures of three species - Longbilled Vulture, White-rumped Vulture, and Slender-billed Vulture - at the BNHS-Vulture Conservation Breeding Centre, Pinjore, Haryana. Cloacal and choanal swabs from 17 captive vultures, hatched at the Centre, were collected and analyzed for aerobic culturable bacteria. The birds were hatched in captivity and were in captivity from hatching till the time of sampling. Results from these samples were compared with the patterns obtained from 32 wild hatched vultures held in captivity at the centre, with patterns from four free ranging Red-headed Vultures used as control. No significant differences were found between the microflora of the captive and wild hatched vultures held in captivity, thus indicating that captivity did not affect the microflora of the captive hatched vultures. This study is the first of a series of planned studies, to ensure that the captive hatched vultures remain healthy and fit to be released in the wild.References
Ali, S. & S.D. Ripley (1983): Handbook of the Birds of India and Pakistan. Compact edition. Oxford University Press, New Delhi.
Bangert, R.L., A.C.S. Ward, E.H. Stauber, B.R. Cho & P.R. Widders (1988): A survey of the aerobic bacteria in the feces of captive raptors. Avian Diseases 32: 53-62.
Blanco, G., J.A. Lemus & J. Grande (2006): Faecal bacteria associated with different diets of wintering red kites: influence of livestock carcass dumps in microflora alteration and pathogen acquisition. Journal of Applied Ecology 43(5): 990-998.
Blanco, G., J.A. Lemus, J. Grande, L. Gangoso, J.M. Grande, J.A. Donazar, B. Arr oyo, O. Frias & F. Hiraldo (2007): Geographical variation in cloacal microflora and bacterial antibiotic resistance in a threatened avian scavenger in relation to diet and livestock farming practices. Environmental Microbiology 9(7): 1738-1749.
Dhanasiri, A., L. Brunvold, M. Brinchmann, K. Korsnes, O. Bergh & V. Kiron (2011): Changes in the intestinal microbiota of wild Atlantic Cod Gadus morhua L. upon captive rearing. Microbial Ecology 61: 20-30.
Green, R.E., I. Newton, S. Shultz, A.A. Cunningham, M. Gilbert, D.J. Pain & V. Prakash (2004): Diclofenac poisoning as a cause of vulture population declines across the Indian subcontinent. Journal of Applied Ecology 41(5): 793-800.
Holt, J.G., N.R. Krieg, P.H.A. Sneathm & J.T. Staley (1984): Bergey’s Manual of Determinative Bacteriology. 9th edn. Williams & Williams, Baltimore, M.D.
Kocijan, I., R.E. Prukner , R. Beck, A. Galov, A. Marinculic & G. Susic (2009): Microflora and intestinal parasites of the Eurasian Griffon vultures (Gyps fulvus) in Croatia. European Journal of Wildlife Research 55: 71-74.
Ley, R.E., M. Hamady, C. Lozupone, P.J. Turnbaugh, R.R. Ramey, J.S. Bircher, M.L. Schlegel, T.A. Tucker, M.D. Schrenzel, R.
Knight & J.I. Gordon (2008): Evolution of mammals and their gut microbes. Science 320(5883): 1647-1651.
Mcfall-Ngai, M., M.G. Hadfield, T.C. Bosc h, H.V. Carey, T. DomazetLošo, A.E. Douglas, N. Dubilier, G. Eberl, T. Fukami, S.F. Gilbert, U. Hentschel, N. King, S. Kjelleberg, A.H. Knoll, N. Kremer, S.K. Mazmanian, J.L. Metcalf, K. Nealson, N.E. Pierc e, J.F. Rawls, A. Reid, E.G. Ruby, M. Rumpho, J.G. Sanders, D. Tautz & J.J. Wernegreen (2013): Animals in a bacterial world, a new imperative for the life sciences. Proceedings of the National Academy of Sciences USA 110(9): 3229-3236.
Nelson, T.M., T.L. Rogers, A.R. Carlini & M.V. Brown (2013): Diet and phylogeny shape the gut microbiota of Antarctic seals: a comparison of wild and captive animals. Environmental Microbiology 15(4): 1132-1145.
Oaks , J.L., M. Gilbert, M.Z. Virani, R.T. Watson, C.U. Meteyer, B.A. Rideout, H.L. Shivaprasad, S. Ahmed, M.J. Chaudhry, M. Arshad, S. Mahmood, A. Ali & A.A. Khan (2004): Diclofenac residues as the cause of vulture population decline in Pakistan. Nature 427(6975): 630-633.
Prakash, V., M.C. Bishwakarm a, A. Chaudhary, R. Cuthbert, R. Dave, M. Kulkarni, Sashi Kumar, K. Paudel, S. Ranade, R. Shringarpure & R.E. Green (2012): The population decline of Gyps vultures in India and Nepal has slowed since veterinary use of diclofenac was banned. PloS ONE 7(11): e49118. doi:10.1371/ journal.pone.0049118.
Quinn, P.J., M.E. Carter, B.K. Mark ey & G.R. Carter (1994): Clinical Veterinary Microbiology. Wolfe Publishing, London.
Ridaura, V.K., J.J. Faith, F.E. Rey, J. Cheng, A.E. Duncan, A.L. Kau, N.W. Griff in, V. Lomb ard, B. Henriss at, J.R. Bain, M.J. Muehlbauer, O. Lkayeva, C.F. Semenkovich, K. Funai, D.K. Hayashi, B.J. Lyle, M.C. Martini, L.K. Urs ell, J.C. Clemente, W.V. Treuren, W.A. Walters , R. Knight, C.B.Newgard, A.C. Heath & J.L. Gordon (2013): Gut microbiota from twins discordant for obesity modulate metabolism in mice. Science 341(6150): 1241214-1 to 1241214-10. doi: 10.1126/ science.1241214.
Scupham, A.J., T.G. Patton, E. Bent & D.O. Bayles (2008): Comparison of the cecal microbiota of domestic and wild turkeys. Microbial Ecology 56(2): 322-331.
Shringarpure, R.N., M.D. Kulkarni, C. Sawant, A. Bhagwat, T.H. Galligan & V. Prakash (2014): Aerobic culturable microflora in resident Gyps vulture species of India. J. Bombay Nat. Hist. Soc. 111(1): 29-35. doi: 10.17087/bnhs/2014/v111i1/56524.
Silva, N., G. Igrejas, A. Gonzalves & P. Poeta (2011): Commensal gut bacteria: distribution of Enterococcus species and prevalence of Escherichia coli phylogenetic groups in animals and humans in Portugal. Annals of Microbiology 62(2): 449-459. doi: 10.1007/ s13213-011-0308-4.
Todar, K. (2008): The normal bacterial flora of humans. In: Todar’s online textbook of bacteriology. Retrieved from http:// textbookofbacteriology.net/normalflora.html
Uenishi, G., S. Fujita, G. Ohashi, A. Kato, S. Yamauchi, T. Matsuzawa & K. Ushida (2007): Molecular analyses of the intestinal microbiota of chimpanzees in the wild and in captivity. American Journal of Primatology 69(4): 367-376. doi: 10.1002/ajp.20351.
Villers , L.M., S.S. Jang, C.L. Lent, S.C. Lewin-Koh & J.A. Norosoarinaivo (2008): Survey and comparison of major intestinal flora in captive and wild Ring-tailed Lemur (Lemur catta) populations. American Journal of Primatology 70(2): 175-184.
Wienemann, T., G. Schmitt-Wagner, K. Meuser, G. Segelbacher, B. Schink, A. Brune & P. Berthold (2011): The bacterial microbiota in the ceca of Capercaillie (Tetrao urogallus) differs between wild and captive birds. Systematic and Applied Microbiology 34(7): 542-551. doi: 10.1016/j.syapm.2011.06.003.
Winsor, D.K., A.P. Bloebaum & J.J. Mathewson (1981): Gram-negative, aerobic, enteric pathogens among intestinal microflora of wild Turkey vultures (Cathartes aura) in West Central Texas. Applied and Environmental Microbiology 42(6): 1123-1124.
Xenoulis, P.G., P.L. Gray, D. Brightsmith, B. Palculict, S. Hoppes, J.M. Steiner, I. Tizard & J.S. Suchodolski (2010): Molecular characterization of the cloacal microbiota of wild and captive parrots. Veterinary Microbiology 146(3-4): 320-325. doi: 10.1016/j.vetmic.2010.05.024.
Zupancic, M.L., B.L. Cantarel, Z. Liu, E.F. Drabek, K.A. Ryan, S. Cirimotich, C. Jones, R. Knight, W.A. Walters, D. Knights, E.F. Mongodin, R.B. Horenstein, B.D. Mitchell, N. Steinle, S. Snitker, A.R. Shuldiner & C.M. Fraser (2012): Analysis of the gut microbiota in the old order Amish and its relation to the metabolic syndrome. PLoS ONE 7(8): e43052. doi: 10.1371/journal.pone.0043052.